+44 (0) 1525 382 277
0
Your Basket
Agarose LE, Organic Solvent Free
#CLS-1910

AGAROSE LE, ORGANIC SOLVENT-FREE

 

• Multi-Purpose, High Purity

• Enhanced Resolution and Clarity

• Reduced Background

• Low EEO / Increased Electrophoretic Mobility

• RNase-, DNase- and Protease-Free

• High Hysteresis / Improved Thermal Stability

 

Agarose LE is a highly purified agarose, used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols.  It's refined using a process that excludes organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150bp to 6kbp).

 

 Its low EEO (Less than or Equal to 0.13mr) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counter flow) that can result from the presence of excessive sulfate-rich negative ions.

 

Agarose LE easily soluble and free of nucleases. It is highly transparent (forms a clear, colorless solution at 1g: 100mL H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.

 

Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added.

 

 Specifications

Moisture: Less than or Equal to 7%

EEO (Electroendosmosis): Less than or Equal to 0.13-mr

Sulfate: Less than or Equal to 0.20%

Gelling Temp. (at 1.5%): 36° ± 1.5°C

Melting Temp. (at 1.5%): 88° ± 1.5°C

Gel Strength (at 1.0%): 1,200g/cm2

Gel Strength (at 1.5%): 2,500g/cm2

DNase, RNase: None Detected

Endonuclease: None Detected

Protease: None Detected

Store At: Room Temperature

 

Name / Number Size Basket
CLS-1910-025 25g
CLS-1910-100 100g
CLS-1910-500 500g

Featured

New E3 Drying Cabinets

NEW E3 Drying Cabinets Our new Sustainable E3 range of glassware drying cabinets, now distributed by GPE Scientific are unique - first to market, energy efficient, safer and cheaper to run. Working with the University of Cambridge, the E3 is a novel, sustainable solution to glassware drying, In addition they’re much easier to use. The E3 drying cabinets are suitable for general purpose warming applications and drying glassware or instruments. All models in this range are fully insulated for improved efficiency and energy savings. Made from sheet steel finished in an easy to clean powder coated paint, with stainless steel interior. Fitted with adjustable shelf runners, removable chrome-plated wire grid shelves and ventilation outlet. Heated by Incoloy sheathed elements in the base of the unit. All units have toughened glass doors; 100 and 200 litre models have sliding doors and the 425 and 885 litre models have double-glazed hinged doors. The 425 and 885 litre models are fitted with lockable castors. Energy efficient, safe, sustainable. Low Energy and part of our NEW Sustainable Solutions Divsion Timer to automate shut down periods Fully insulated with adjustable vent cover Integral 7 Day time with display  Digital Controller with fixed over-temperature cut-out Adjustable shelf runners with removeable chrome shelves Lockable castors available on the 425 & 885 litre models Easy to clean powder coated body with toughened glass doors Benefits & Cost Savings: Low Energy consumption - >50% lower than traditional cabinets! Low heat output - reducing air conditioning costs Fully insulated - safer for the end user Easy, specific temperature controller  Integral programmable 7 day timer - on/off to reflect your needs Convection or fan - heat up time <1 hour
  Find out more

Request a quote

To request a quote simply add the product you are interested in to your basket, fill in the contact form and we will get back to you with a price.
  View quote basket