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Agarose LE, Organic Solvent Free
CLS-1910

AGAROSE LE, ORGANIC SOLVENT-FREE

 

• Multi-Purpose, High Purity

• Enhanced Resolution and Clarity

• Reduced Background

• Low EEO / Increased Electrophoretic Mobility

• RNase-, DNase- and Protease-Free

• High Hysteresis / Improved Thermal Stability

 

Agarose LE is a highly purified agarose, used for nucleic acid electrophoresis (analytical and preparative), protein electrophoresis (including radial immunodiffusion) and various blotting protocols.  It's refined using a process that excludes organic solvents, yielding a cleaner end-product with a significantly reduced environmental impact. Agarose LE can be used for analyses of proteins and nucleic acids of various sizes (150bp to 6kbp).

 

 Its low EEO (Less than or Equal to 0.13mr) promotes increased electrophoretic mobility, yielding improved resolution and shorter run times. This also allows macromolecules and larger particles (subcellular fragments, viruses, etc.) to migrate more freely through the gel matrix. The consistently low EEO also provides a reduction in band distortion (caused by counter flow) that can result from the presence of excessive sulfate-rich negative ions.

 

Agarose LE easily soluble and free of nucleases. It is highly transparent (forms a clear, colorless solution at 1g: 100mL H2O), and exhibits exceptionally low absorption of chemical staining agents. Pore size can be adjusted by simple modifications to the concentration ratio.

 

Formulated for high gel strength and integrity, Agarose LE exhibits exceptional thermal stability and mechanical resistance, ensuring safe, easy handling, regardless of whether a denaturing agent has been added.

 

 Specifications

Moisture: Less than or Equal to 7%

EEO (Electroendosmosis): Less than or Equal to 0.13-mr

Sulfate: Less than or Equal to 0.20%

Gelling Temp. (at 1.5%): 36° ± 1.5°C

Melting Temp. (at 1.5%): 88° ± 1.5°C

Gel Strength (at 1.0%): 1,200g/cm2

Gel Strength (at 1.5%): 2,500g/cm2

DNase, RNase: None Detected

Endonuclease: None Detected

Protease: None Detected

Store At: Room Temperature

 

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Product Code Size Basket
CLS-1910-025 25g
CLS-1910-100 100g
CLS-1910-500 500g
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