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CLARIOstar Plus

Most flexible Plate Reader for Assay Development
The new CLARIOstar® Plus is the latest addition to our family. Our most flexible multi-mode plate reader comes equipped with our patented LVF Monochromators™, filters, and spectrometer.

Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development.

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  • Innovative monochromators for increased sensitivity
  • Fully-flexible wavelengths and bandwidths
  • Spectral scanning in ABS, FI and LUM
  • Largest dynamic range with no manual intervention
  • Rapid full-plate autofocus
  • Dedicated features for live cell-based assays 
  • Ultra-fast data sampling (100 measurements/second)
  • Dedicated laser for Alpha Technology
More details:
Detection Modes
Fluorescence intensity (incl. FRET)

Fluorescence polarization 


Luminescence (flash and glow) - incl. BRET

Time-resolved fluorescence


UV/vis absorbance spectra

Measurement Modes
Top and bottom reading

Endpoint and kinetic

Sequential multi-excitation

Sequential multi-emission

Spectral scanning (fluorescence, luminescence, absorbance)

Ratiometric measurements

Well scanning

Microplate Formats
6- to 1536-well plates, user-definable

LVis Plate with 16 low volume microspots (2 μL)

Microplate Carrier
Robot compatible

Light Sources
High energy xenon flash lamp

Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM

Low-noise photomultiplier tube

Red-shifted photomultiplier tube

CCD spectrometer

Wavelength Selection
Dual Linear Variable Filter (LVF) Monochromators™

Linear Variable Dichroic Mirror

Separates ex & em LVF Monochromators

Optical filters:

Ex and em slides hold 4 filters each

LVF Monochromators + optical filters

Use one for ex and the other for em 

UV/Vis absorbance spectrometer:

Full spectra or 8 distinct wavelengths in < 1 sec/well

Optical Filters
Excitation and emission slides for 4 filters each

Optical Path Guides
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics

Automatic focal height adjustment (0.1 mm resolution)

Spectral Range
FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT)
LUM: 240 - 740 nm
LVF Monochromators™
FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT)
LUM: 320 - 740 nm
Linear Variable Dichroic
340 - 740 nm or 340 - 760 nm (red-shifted PMT)
ABS: 220 - 1000 nm
FI filters
< 0.15 pM (< 3 amol/well FITC, 384sv, 20 μL)
FI filters
< 1.0 pM (< 50 amol/well FITC, 384g, 50 μL)
FI monochromator

< 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL)
FI monochromator
< 3.0 pM (< 150 amol/well FITC, 384g, 50 μL)

Dynamic Range: 8 decades in a single measurement

< 0.5 mP SD at 1 nM FITC (384sv, 20 μL)
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 880 % (High Calibrator)
Delta F > 30 % (Low Calibrator)
< 20 fM europium, 384, 80 μL
< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL)
Dynamic range: 8 decades in a single measurement
with laser

< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
with spectrometer
Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Read Times
Flying mode (1 flash)
8 s (96), 15 s (384), 28 s (1536)
10 flashes
19 s (96), 57 s (384), 3 min 4 s (1536)
Reagent Injection
Up to 2 built-in reagent injectors
Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
Variable injection speed up to 420 µL / s
Reagent back flushing

Linear, orbital, and double-orbital with user-definable time and speed

Integrated Barcode Reader
Up to two integrated barcode readers

+3 °C above ambient up to 45 °C or 65 °C

The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.

Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant

Integrated fluorophore library

Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg

LVis Plate
Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.

Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy

Dimensions: conforms to SBS standards for microplates.

Atmospheric Control Unit (ACU)
Actively regulates O2 and CO2 - 0.1-20%

Magazines for up to 50 plates – continuous loading feature

Microplate incubator and shaker

Optical Filters
Excitation and emission filters

The CLARIOstar Plus can include all or any combination of features listed below at purchase. Upgrading with additional features is possible at any time. Please contact your local representative for more details or a quote.

LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates

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