Detection Modes
Fluorescence intensity (incl. FRET)
Fluorescence polarization
AlphaScreen®/AlphaLISA®/AlphaPlexTM
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/vis absorbance spectra
Measurement Modes
Top and bottom reading
Endpoint and kinetic
Sequential multi-excitation
Sequential multi-emission
Spectral scanning (fluorescence, luminescence, absorbance)
Ratiometric measurements
Well scanning
Microplate Formats
6- to 1536-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
Microplate Carrier
Robot compatible
Light Sources
High energy xenon flash lamp
Dedicated laser for AlphaScreen®/AlphaLISA®/AlphaPlexTM
Detectors
Low-noise photomultiplier tube
Red-shifted photomultiplier tube
CCD spectrometer
Wavelength Selection
Dual Linear Variable Filter (LVF) Monochromators™
Linear Variable Dichroic Mirror
Separates ex & em LVF Monochromators
Optical filters:
Ex and em slides hold 4 filters each
LVF Monochromators + optical filters
Use one for ex and the other for em
UV/Vis absorbance spectrometer:
Full spectra or 8 distinct wavelengths in < 1 sec/well
Optical Filters
Excitation and emission slides for 4 filters each
Optical Path Guides
Top and bottom: free-air optical light path guided by motor-driven mirrors and dichroics
Z-Adjustment
Automatic focal height adjustment (0.1 mm resolution)
Spectral Range
Filters
FI, FP, TRF: 240 - 740 nm or 240 - 900 nm (red-shifted PMT)
LUM: 240 - 740 nm
LVF Monochromators™
FI: 320 - 740 nm or 320 - 840 nm (red-shifted PMT)
LUM: 320 - 740 nm
Linear Variable Dichroic
340 - 740 nm or 340 - 760 nm (red-shifted PMT)
Spectrometer
ABS: 220 - 1000 nm
Sensitivity
FI filters
(top)
< 0.15 pM (< 3 amol/well FITC, 384sv, 20 μL)
FI filters
(bottom)
< 1.0 pM (< 50 amol/well FITC, 384g, 50 μL)
FI monochromator
(top)
< 0.35 pM (< 7 amol/well FITC, 384sv, 20 μL)
FI monochromator
(bottom)
< 3.0 pM (< 150 amol/well FITC, 384g, 50 μL)
Dynamic Range: 8 decades in a single measurement
FP
< 0.5 mP SD at 1 nM FITC (384sv, 20 μL)
HTRF®
(black and white microplates)
Reader Control Kit (Eu) after 18h (384sv, 20 μL)
Delta F > 880 % (High Calibrator)
Delta F > 30 % (Low Calibrator)
TRF
< 20 fM europium, 384, 80 μL
LUM
< 0.4 pM (< 8 amol/well ATP, 384sv, 20 μL)
Dynamic range: 8 decades in a single measurement
AlphaScreen®
with laser
< 5 pM (< 100 amol/well P-Tyr-100, 384sv, 20 μL)
ABS
with spectrometer
Full spectrum captured in < 1 s / well
Selectable spectral resolution: 1, 2, 5, and 10 nm
OD range: 0 - 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Read Times
Flying mode (1 flash)
8 s (96), 15 s (384), 28 s (1536)
10 flashes
19 s (96), 57 s (384), 3 min 4 s (1536)
Reagent Injection
Up to 2 built-in reagent injectors
Individual injection volumes for each well: 3 to 500 µL (optionally up to 2 mL)
Variable injection speed up to 420 µL / s
Reagent back flushing
Shaking
Linear, orbital, and double-orbital with user-definable time and speed
Integrated Barcode Reader
Up to two integrated barcode readers
Incubation
+3 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
Software
Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
Integrated fluorophore library
Dimensions
Width: 45 cm, depth: 51 cm, height: 40 cm; weight: 32 kg
LVis Plate
Sample capacity: sixteen separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.
Quality Control Internal Standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates.
Atmospheric Control Unit (ACU)
Actively regulates O2 and CO2 - 0.1-20%
Stacker
Magazines for up to 50 plates – continuous loading feature
THERMOstar
Microplate incubator and shaker
Optical Filters
Excitation and emission filters
Upgrades
The CLARIOstar Plus can include all or any combination of features listed below at purchase. Upgrading with additional features is possible at any time. Please contact your local representative for more details or a quote.
*
LOD = 3 x SD (20 blanks) / slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384sv = 384-well small volume microplates
384g = 384-well glass bottom microplates
1536 = 1536-well microplates